Self-Condensation Culture Enables Vascularization of Tissue Fragments for Efficient Therapeutic Transplantation

Posted on Posted in AMRC Research

Date: 9 May 2018

 

Highlights
  • Self-condensation culture enables endothelialization of diverse tissue fragments
  • Vascularization facilitates tissue survival after transplantation
  • Vascularized islet transplant enhances therapeutic potential against diabetes
  • Rapid induction of functional vasculatures preserves original islet architecture

 

Summary

Clinical transplantation of tissue fragments, including islets, faces a critical challenge because of a lack of effective strategies that ensure efficient engraftment through the timely integration of vascular networks. We recently developed a complex organoid engineering method by “self-condensation” culture based on mesenchymal cell-dependent contraction, thereby enabling dissociated heterotypic lineages including endothelial cells to self-organize in a spatiotemporal manner. Here, we report the successful adaptation of this method for generating complex tissues from diverse tissue fragments derived from various organs, including pancreatic islets. The self-condensation of human and mouse islets with endothelial cells not only promoted functionalization in culture but also massively improved post-transplant engraftment. Therapeutically, fulminant diabetic mice were more efficiently treated by a vascularized islet transplant compared with the conventional approach. Given the general limitations of post-transplant vascularization associated with 3D tissue-based therapy, our approach offers a promising means of enhancing efficacy in the context of therapeutic tissue transplantation.

 

Link to full article

 

For inquiries regarding this press release

Takanori TAKEBE

E-mail: ttakebe@yokohama-cu.ac.jp

 

Hideki TANIGUCHI

E-mail: rtanigu@yokohama-cu.ac.jp

 

Department of Regenerative Medicine,
Yokohama City University School of Medicine

Fukuura 3-9, Kanazawa-ku, Yokohama, 236-0004 JAPAN