YOKOHAMA CITY UNIVERSITY

Fourth Day of the Science Summer Program

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Lecture 8 - 10 Practice in Advanced Biosciences

Associate Profs. Tomomi Sato and Shinsukev Kutsuna, Prof. Yasuhiro Ozeki, Department of Nano Biosciences

The lectures of the 4th day were separately delivered to 3 teams at the YCU Kanazawa Hakkei Campus.

Immunological detection of hormone-producing cells

The team of Associate Prof. Sato, who is actively involved in research on sex hormones, practiced detecting sex hormones.

A hormone referred to as estrogen is produced by the ovaries. Following the explanation concerning hormones, the ovaries of a mouse were stained by means of antigen-antibody response to enable visualization of estrogens. The picture below (right) shows the stained ovaries. The hormone-producing sites are observed to have been stained red.

While waiting for the next experiment, the students observed the mouse testis and ovary. They then visited Associate Prof. Sato's laboratory, where the students of Associate Prof Sato's team were seen conducting experiments. The students became friends with the members of the team and asked them what experiments were being conducted.

Imaging of recombination proteins using fluorescent protein genes

Practice in the imaging of proteins was conducted by the Kutsuna team. Imaged in this practice were proteins made fluorescent by fluorescent gene-inserted indigo algas and shepherd's purses.
The genes expressed by fluorescent jellyfish genes emit fluorescent light if reagents are added and special light is applied. If genes have been so recombined as to simultaneously express fluorescent genes and protein-expressing genes, proteins are able to be detected by fluorescence. The above picture (in the right column) shows fluorescence being confirmed with instruments.

Chlorophyll was also isolated and detected from plants with which the students are familiar.

Isolation of animal lectins and electrophoresis

The students of the team led by Prof. Ozeki conducted an exercise showing proteins with avidity for sugar chains.

Lectins are present in every animal. For research, Prof. Ozeki uses lectins originating in marine invertebrates, so an observation was made by extracting lectins from specimens.

Lectins were extracted as follows: specimens were grounded up; centrifuged; sifted in a gel condition and lectins were extracted.

Finally, reactions shown by blood, body fluid and various substances were observed.

Pictures show the manipulation of a centrifugal separator and affinity chromatography.
Erythrocytes are observed.
Cell congregation occurred when lectins were mixed.