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Department of Reserch Support and coordination
Message from the Department Director

In order to conduct research efficiently, we need to organize core-facility. We coordinate seven core-facility centers: human tissue processing, animal-model of disease, genome, proteome, cellome, imaging, and bio-bank. Each center is described briefl y as follows:
1 ) Human tissue processing center treats exclusively human tissues to prevent infection.
2 ) Animal-model of disease center establishes disease model with genetic techniques.
3 ) Genome center focuses on identifi cation of mutations in the genome of genetically inherited disease with high-speed sequencer (eg. next generation sequencer).
4 ) Proteome center analyzes protein modifi cations related to disease with mass spectrometry.
5 ) Cellome center aims to innovate new treatment of disease with the manipulation at the cellular level.
6 ) Imaging center coordinates state-of-art imaging apparatuses (two photon laser scanning microscope, electron microscope, etc.) and aims to elucidate mechanisms underlying disease.
7 ) Bio-bank collects and stores human tissue samples obtained from patients.
Director, Department of Research Support and Coordination
Professor, Department of Physiology, Graduate School of Medicine
TAKUYA TAKAHASHI
Biobank Division
It is essential for promoting TR(translational research) to evaluate whether the fi ndings obtained from an animal and tissue culture experiment are actually true in human beings. Human tissue and blood sample obtained at the surgical and diagnostic procedures are necessary for such research. In the past, the research using human samples were limited to clinicians. These days, however, research methods have improved so much that even basic medical researchers can perform such tasks.
But, unfortunately there still remain certain distancebetween the basic scientists and the hospital clinicians.
Our biobank was established as an infrastructre of TR to bridge between the scientists and clinical doctors in October 2006. With appropriate informed consents, the biobank stores human samples collected from healthy volunteers, as well as from patients in our university hospital facility. We constructed an appropriate system to avoid the disadvantage of the patients. At present five clinical departments participate in this system.
Until October 2009, 4,621 tubes of samples from 566 patientsare stored in the deep freezer of the biobank.
Several other medical schools are now conducting the plan of biobank.
Genome Analyses Division
Section for genetic and genomic analyses. Highly advanced technologies such as next generation sequencer are available to develop innovative methods to diagnose and predict human diseases for the ordermade medicine.
* Main Equipments
Illumina Genome Analyzer II
ABI3500xl Genetic Analyzer
Agilent DNA Microarray Scanner G2565CA
Proteome Analyses Division
Advanced modern instruments including mass spectrometers are well equipped for protein or proteome analyses in this laboratory. Using these instruments, we
can detect and identify proteins associated with cancers and life-style related diseases, and verify if they can be utilized as early diagnosis markers or drug discovery targets.
* Main Equipments
Mass spectrometer (MALDI-TOF/TOF MS, ESI-Q/TOF MS, ESI-QIT MS,ESI-LTQ Orbitrap MS)
High performance liquid chromatography
Laser microdissection
Cellome Analyses Division
This laboratory conducts analyses at the cellular level. Fully furnished with cell sorters and other modern equipment for cutting-edge cell biology, our laboratory carries out research on the basis of human stem cell separation/manipulation and genetic engineering technologies. Our goal is to develop innovative treatments, under the general framework of "gene therapy and regenerative medicine" for cancer, lifestylerelated diseases and infectious diseases.
* Main Equipments
Cell Sorter (Jet-in-Air method)
Cell sorter (Flow-Cell method)
Cell Imaging Analyzer
Imaging Division
In this department, we investigate the cellular and molecular dynamics with state-of-the-art imaging techniques (e.g., two photon laser scanning microscopes, and an electron microscope.) Our goal is to innovate novel diagnoses and treatments for diseases.
* Main Equipments
Two photon laser scanning microscope
Electron microscope
Confocal laser scanning microscope
Human Tissue Processing Division
This is a facility to process human tissue and bloods for research use. Frozen section, primary culture and purifi cation to DNA, RNA and protein , can be processed here. To avoid risks of contamination with experimental animal samples, as well as to avoid infectious accidents to human samples, materials handled in this division are limited to human samples. To further minimize the risk of cross-contamination, air pressure of each room is set to "negative."
* Main Equipments
Safety Cabinet
CO2 Incubator
Refrigerated Centrifuge
Animal Model Analyses Division
Structural and functional analyses of cells and tissues sampled from animal disease model mice and rats are specialized in this division.
* Main Equipments
Laser Microdisection
Real-time PCR
Ultramicrotome
